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Technical Support Team
Branch Chief :
Kyungjae Myung, PhD, Director, IBS-CGI
Members of CORE
Sunyoung Hwang
EunA Lee
Jaesun Ra
In Bae Park
Hyejin Kim
Research Fellow :
Jun Hong Park, PhD
Tae Moon Kim, PhD
Principal Researcher :
Byung-Gyu Kim, PhD
Jung me Hwang, PhD
We are Core technical support team for the IBS Center for Genomic Integtiry. Unlike other members of CGI, we do not belong to a specific branch, but help everybody with their research. Our awesome and incredible team can help you with a number of special assays:
Sunyoung Hwang: iPOND & Silac iPOND
EunA Lee: DNA combing assay & HR assay
Jaesun Ra: various telomere and chromosome assays, cell sorting
In Bae Park: Genotyping and mouse facility management
Byung-gyu Kim: Mass spectrometry and proteomics
Jung me Hwang: Equipment and lab management
To get the best possible results, we can help you with the use our equipment that helps you with your research. (Molecular Combing System, Microscopy, Mass spectrometry and FACS etc.). We do not only cooperate with researcher at our center but also with research groups at UNIST to apply our skills. Please contact us to take advantage of the technical support, we can offer.
Major Equipment Brochure IRIS
Super sensitive high resolution confocal laser scanning microscope for live cell
System operator:
Dr. Jung Me Hwang , Senior technician
E: hjm072@ibs.re.kr T: +82-10-7749-7427
![LSM 880 with Airyscan [Carl Zeiss / Germany]](/img/cgi_en/contents/img_major_con1.jpg)
LSM 880 with Airyscan [Carl Zeiss / Germany]
LSM 880 with Airyscan has a technique for obtaining high-resolution optical images with depth selectivity. Images are acquired point by-point and reconstructed with a computer, allowing three-dimensional reconstructions of complex objects. With Airyscan, the revolutionary detection concept from ZEISS, you can use multicolor samples with any label and get image quality like you have never seen before. You are always able to select the optimal acquisition strategy for your sample: Simply decide whether you want to gain 1.7x higher resolution in all three dimensions resulting in a 5x smaller confocal volume. Or push the sensitivity beyond the limits of all conventional confocals. Or use the increase in signal-tonoise ratio to speed up your image acquisition.
Application
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· Super-Resolution Live Cell Imaging with standard multi-color live samples
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· Super-Resolution at high penetration depth for large and thick samples
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· UV Irradiation : for Detection of DNA recovery
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· Tile Scanning : for experiment of DNA fiber / Combing assay
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· Fixed Cell Imaging for Immunostaining & linear scanning
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· Detection of cell cycle during long-term live cell
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· Protein-Protein Interaction with CUPID system
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· ROI-HDR, FRET, FRAP, FCS and 3D Visualization
High Resolution Mass Spectrometer analysis facility
System operator:
Dr. Byung-Gyu Kim , Senior technician
E: goldenlion@ibs.re.kr T: +82-10-7192-7638
![Orbitrap Fusion Lumos [Thermo Fisher Scientific ]](/img/cgi_en/contents/img_spectrom_con1.jpg)
Orbitrap Fusion Lumos [Thermo Fisher Scientific ]
The Orbitrap Fusion Lumos Tribrid Mass Spectrometer is designed to expand performance in advanced proteomics, biopharma and metabolomics applications, including quantitation using isobaric tags, low level PTM analysis, data independent acquisition (DIA), and top down proteomics. The new instrument features enhanced sensitivity resulting in improved analyte detection, characterization and quantitation, enabling scientists to perform more comprehensive sample analyses faster and with better accuracy than ever before. The system is the research-leading high performance mass spectrometer with enhanced sensitivity facilitated by a new High Capacity Transfer Tube, Electrodynamic Ion Funnel, Advanced Quadrupole Technology, Advanced Vacuum Technology, and ETD HD.
Proteomics based DNA integrity research
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· Affinity purification (AP)-MS based DNA integrity regulators interactome profiling
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· Post-translational modification (PTM) identification of DNA integrity regulators
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· Monitoring protein dynamics at replication fork with SILAC-iPOND analysis
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· DNA integrity linked drug target identification
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· Differential expressed protein (DEP) profiling
Tel. +52-217-5529 / Fax. +52-217-5519
Copyright(c) 2014 Center for Genomic Integrity at IBS. All Rights Reserved.