Center for Genomic Integrity

The Molecular and Cellular Biology Branch (MCBB) of IBS-CGI investigates various molecular mechanisms affecting genomic integrity; DNA replication, repair, recombination and damage response. We use molecular, cellular biology, and biochemistry methods to understand detail molecular mechanisms affecting genomic integrity. In particular, the MCBB focuses on mammalian DNA replication during cellular proliferation and under stress conditions. The MCBB uses mouse and zebrafish as model organisms to understand phenotypic consequences when genomic integrity is challenged genetically or by DNA damaging agents. Lastly, the MCBB characterizes small molecules affecting different DNA repair pathways. These multidisciplinary researches in the MCBB will elucidate detail molecular mechanisms responsible for preventing tumorigenesis and aging. Currently, the MCBB has multiple collaborations with national and international laboratories.

Research in the Chemical and Cancer Biology Branch of IBC-CGI focuses on DNA repair and damage response pathways and their relationship to carcinogenesis and anti-tumor therapy. Biochemical, molecular and cell biology studies are combined with organic chemistry to study the molecular mechanisms of pathways such as nucleotide excision repair, interstrand crosslink repair and responses to replication stress. Our studies aim to elucidate the mechanisms of the pathways preventing tumorigenesis and inherited genetic disorders xeroderma pigmentosum and Fanconi anemia. We are also interested in how DNA repair capacity in tumors may be assessed and/or targeted for inhibition to improve therapeutic outcomes by agents such as cisplatin. Our research is inherently multi-disciplinary and involves national and international collaborations with many laboratories.

We are Core technical support team for the IBS Center for Genomic Integtiry. Unlike other members of CGI, we do not belong to a specific branch, but help everybody with their research. Our awesome and incredible team can help you with a number of special assays:
Sunyoung Hwang: iPOND & Silac iPOND
EunA Lee: DNA combing assay & HR assay
Jaesun Ra: various telomere and chromosome assays, cell sorting
In Bae Park: Genotyping and mouse facility management
Byung-gyu Kim: Mass spectrometry and proteomics
Jung me Hwang: Equipment and lab management

To get the best possible results, we can help you with the use our equipment that helps you with your research. (Molecular Combing System, Microscopy, Mass spectrometry and FACS etc.). We do not only cooperate with researcher at our center but also with research groups at UNIST to apply our skills. Please contact us to take advantage of the technical support, we can offer.

Application

• · Super-Resolution Live Cell Imaging with standard multi-color live samples

• · Super-Resolution at high penetration depth for large and thick samples

• · UV Irradiation : for Detection of DNA recovery

• · Tile Scanning : for experiment of DNA fiber / Combing assay

• · Fixed Cell Imaging for Immunostaining & linear scanning

• · Detection of cell cycle during long-term live cell

• · Protein-Protein Interaction with CUPID system

• · ROI-HDR, FRET, FRAP, FCS and 3D Visualization

Proteomics based DNA integrity research

• · Affinity purification (AP)-MS based DNA integrity regulators interactome profiling

• · Post-translational modification (PTM) identification of DNA integrity regulators

• · Monitoring protein dynamics at replication fork with SILAC-iPOND analysis

• · DNA integrity linked drug target identification

• · Differential expressed protein (DEP) profiling